Table 1 List of primer pairs used in the current study for amplification of EP receptor isoforms.
From: Role of cAMP in the promotion of colorectal cancer cell growth by Prostaglandin E2
EP receptor | Acc. No. | sense | antisense | fragment size in bp | Ref |
|---|---|---|---|---|---|
EP1 | NM_000955 | CTTGTCGGTATCATGGTGGTGTC | GGTTGTGCTTAGAAGTGGCTGAGG | 322 | [27] |
EP2 | NM_000956 | CCACCTCATTCTCCTGGCTA | CGACAACAGAGGACTGAACG | 216 | [26] |
EP3 subtype 1–8 | NM_000957 | CTTCGCATAACTGGGGCAAC | TCTCCGTGTGTGTCTTGCAG | 300 | [27] |
EP3 subtype 1–3 | NM_000957 | CTTAATAGCTGTTCGCCTGG | GCTTAGCTGGACACTGCAG | 293 (1) 224 (2) 197 (3) | This study |
EP3 subtype 4 | NM_198716 | CTTAATAGCTGTTCGCCTGG | ATTTCCCCAAAATTCCTCTTG | 232 | This study |
EP3 subtype 5 | NM_198715 | CTTAATAGCTGTTCGCCTGG | TGCTTCTGTCTGTATTATTTCAT | 182 | This study |
EP3 subtype 6 | NM_198716 | CTTAATAGCTGTTCGCCTGG | ATTTCCCCAAAATTCCTCTTG | 140 | This study |
EP3 subtype 7 | NM_198717 | CTTAATAGCTGTTCGCCTGG | ATTTCCCCAAAATTCCTCCTG | 113 | This study |
EP3 subtype 8 | NM_198718 | CTTAATAGCTGTTCGCCTGG | GTCTTTACTGTTGAGATTCTG | 268 | This study |
EP4 | NM_000958 | TGGTATGTGGGCTGGCTG | GAGGACGGTGGCGAGAAT | 329 | [26] |