Skip to main content
Figure 3 | BMC Cancer

Figure 3

From: Role of cAMP in the promotion of colorectal cancer cell growth by Prostaglandin E2

Figure 3

Concentration-dependent effect of PGE2 on cAMP levels in CRC cell lines. A Serum-starved cells were exposed to 100 μM Isoproterenol or the indicated concentrations of PGE2 for 15 min. Cyclic AMP levels were determined as described in the experimental section. B Cyclic AMP-raising agents induce phosphorylation of PKA-substrates in CRC cells. Cells were challenged with 100 μM Isoproterenol or 10 μM forskolin for 20 min and phosphorylation of PKA substrates was determined by western blotting with an antibody directed against the phosphorylated PKA-consensus site. Vinculin levels were determined to ascertain equal loading. C CRC cells were deprived of serum overnight and challenged with varying doses of PGE2 for 15 min. Phosphorylation of PKA substrates was assessed as in B. A densitometric quantification of the signal for the 70–100 kD region is shown. Two additional experiments produced essentially the same results. Two-sample comparisons (all vs. control) were performed with Student's t test. P values ***P < 0,001, **P < 0,01, *P < 0,05.

Back to article page