Table 1 Details of PCR conditions and RFLPs studied
From: Polymorphism +17 C/G in Matrix Metalloprotease MMP8 decreases lung cancer risk
Gene | Polymorphism | Primer sequence | PCR Conditions | Enzyme |
|---|---|---|---|---|
MMP1 | -16071G/2G | (F) TGA CTT TTA AAA CAT AGT CTA TGT TCA (R) TCT TGG ATT GAT TTG AGA TAA GTC ATA gC | 35 cycles: 94°C 30 s, 54°C 30 s, 72°C 30 s | AluI |
MMP8 | + 17C/G | (F) CTG TTG AAG GCC TAG AGC TGC TGC TCC (R) CAT CTT CTC TTC AAA CTC TAC CC | 30 cycles: 94°C 45 s, 64°C 45 s, 72°C 1 min | DdeI |
MMP13 | -77A/G | (F) GAT ACG TTC TTA CAG AAG GGC (R) GAC AAA TCA TCT TCA TCA CC | 30 cycles: 94°C 30 s, 54°C 30 s, 72°C 30 s | BrsI |