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Figure 7 | BMC Cancer

Figure 7

From: tabAnti-HER2 (erbB-2) oncogene effects of phenolic compounds directly isolated from commercial Extra-Virgin Olive Oil (EVOO)

Figure 7

Effects of HER2 siRNA on EVOO polyphenols-induced cytotoxicity. ~5 × 103 cells (96-well plates) overnight serum-starved SKBR3 (left) and MCF-7/HER2 (right) cells were transfected with HER2 siRNA or control siRNA (80 pmols/well) before exposure to 25 μM 1-[+]-acetoxypinoresinol or 25 μM DAOA. The metabolic status of SKBR3 and MCF-7/HER2 cells was evaluated using a MTT-based cell viability assay and Interaction Indexes were calculated by dividing expected cytotoxicities (additive models: sum of cell toxicities induced by each agent alone) by those obtained experimentally in the actual combination (Interaction Indexes < 1, = 1, > 1 < 2, and > 2 denote a nature of the interaction synergistic, additive, antagonistic and protective, respectively). Results are means (dots) and 95% confidence intervals (bars) of three independent experiments made in triplicate.

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