Figure 6

Effects of EVOO phenolics on HER2-enhanced breast cancer cell proliferation. MCF-7/HER2 cells and MCF-7/pBABE matched control cells were incubated with 100 μM of EVOO single phenols (left), fractions containing mainly EVOO lignans (middle) and fractions containing mainly EVOO secoiridoids (right) for 4 days. Cell proliferation, measured using a crystal violet assay as described in "Materials and methods", was expressed as % of untreated cells (dashed line = 100% cell proliferation). Results are means (columns) and 95% confidence intervals (bars) of three independent experiments made in triplicate. Statistically significant differences (one-factor ANOVA analysis) between HER2-negative MCF-7/pBABE control cells and HER2-positive MCF-7/HER2 cells are shown. All statistical tests were two-sided.