Figure 2

Effects of EVOO phenolics on breast cancer cell viability. Figure shows the chromatographic profile of an EVOO sample and the extracted ion of the main phenolics of each fraction. Detection of EVOO phenolics was performed at 280 nm (only in the analysis of elenolic acid – peak number 3 – the detection was performed at 240 nm). The metabolic status of EVOO polyphenols-treated SKBR3 cells was evaluated using a MTT-based cell viability assay and constructing dose-response curves as described in "Materials and methods". Concentrations producing the IC₅₀ value (the concentration of each compound or main compound of the each fraction needed to reduce cell viability by 50% relative to untreated control cells) were calculated by interpolation. Values are means (in μM) and 95% confidence intervals (95% CI) of three independent experiments made in triplicate.