Figure 12

Effects of proteasome inhibition on EVOO polyphenols-induced down-regulation of HER2 protein in breast cancer cells. MCF-7/HER2 cells were exposed to three different schedules: 1.) 1 μM MG-132 (2 hr) → EVOO polyphenols (up to 48 hr); 2.) DMSO (v/v; 2 hr) → EVOO polyphenols (up to 48 hr); 3.) 1 μM MG-132 (2 hr) → EtOH (v/v; up to 48 hr). Oncogene Science HER2 microtiter ELISA was used according to the manufacturer's instructions to compare HER2 protein concentrations in whole cell lysates from schedules #1, #2, and #3. HER2 expression in untreated control cells was set as 100%. Results are means (columns) and 95% confidence intervals (bars) of three independent experiments made in triplicate. Statistically significant differences between experimental conditions and unsupplemented control cells are shown (one-factor ANOVA analysis).