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Figure 11 | BMC Cancer

Figure 11

From: tabAnti-HER2 (erbB-2) oncogene effects of phenolic compounds directly isolated from commercial Extra-Virgin Olive Oil (EVOO)

Figure 11

Influence of EVOO polyphenols on the levels of HER2 tyrosine kinase activity. Overnight serum-starved SKBR3 (left) and MCF-7/HER2 (right) cells were treated with 100 μM 1-[+]-acetoxypinoresinol and 100 μM DAOA for 0, 6, 24 and 48 hr. Oncogene Science HER2 microtiter ELISA was used according to the manufacturer's instructions to compare HER2 protein concentrations in whole cell lysates throughout the time-course. HER2 expression at the 0 hr-time point was set as 100%. Results are means (columns) and 95% confidence intervals (bars) of three independent experiments made in triplicate. Statistically significant differences between experimental conditions and unsupplemented control cells at the 0 hr-time point are shown (one-factor ANOVA analysis). All statistical tests were two-sided. EVOO polyphenols-treated and untreated control cells (0 hr) were tested in parallel for p185HER2 autophosphorylation at Tyr1248 using immunoblotting procedures as described in "Materials and methods".

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