Figure 5
From: Desmoglein 2 is a substrate of kallikrein 7 in pancreatic cancer
Levels of soluble Dsg2 in conditioned medium of KLK7-expressing BxPC-3 cells are higher than in vector-transfected control cells. (A) Levels of soluble Dsg2 (sDsg2) in concentrated conditioned medium from hK7-expressing clones BxPC-3/hK7 clone#3 (lane 1) and clone#7 (lane 2) and vector-transfected clone BxPC-3/Vec (lane 3) were determined by western blot. Sizes of protein markers are indicated on the left. (B) The relative amount of the 80-kDa sDsg2 immunoreactive product was determined for KLK7-transfected (solid bars) and vector-transfected (open bar) BxPC-3 cells by densitometric analysis using Quantity One software and the average relative intensity is depicted. Vertical bars represent SEM (n = 3).