Figure 4

Expression of desmogleins in human pancreatic adenocarcinoma cell lines. (A) Equal amounts of total protein from whole cell lysates of human pancreatic cancer cell lines BxPC-3 (lanes 1 and 3) and Panc-1 (lanes 2 and 4) were separated by SDS-PAGE and visualized by western blot using anti-Dsg1 and anti-Dsg2 antibodies, as indicated. GAPDH levels were monitored as a loading control (arrowhead). Sizes of protein markers are indicated on the left. Appreciable levels of Dsg2 were detected in BxPC-3 cells, but not in Panc-1 cells. Dsg1 was not detected in either of the cell lines. (B) Immunocytochemistry of Dsg2 in BxPC-3 cells using a FITC-conjugated secondary antibody showed specific cell membrane localization. Original magnification x400.