Figure 3
From: Desmoglein 2 is a substrate of kallikrein 7 in pancreatic cancer
Desmoglein 1 and 2 are substrates for hK7 in vitro. Thermolysin-activated hK7 was incubated with recombinant Dsg1 (A) or Dsg2 (B) for the indicated times in the absence (lanes 1–4) or presence (lanes 5–8) of the hK7 inhibitor chymostatin. Reaction products were separated by SDS-PAGE and visualized by western blot using either anti-Dsg1 or anti-Dsg2 antibodies. Sizes of protein markers are indicated on the left.