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Table 1 Antigen expression in melanoma cell lines twenty nine hours following UV and/or gamma-irradiation*

From: The use of gamma-irradiation and ultraviolet-irradiation in the preparation of human melanoma cells for use in autologous whole-cell vaccines

 

% Antigen Expression†

 

gp100

Tyrosinase

MART-1/MelanA

S100

Treatment

DM6

DM93

DM6

DM93

DM6

DM93

DM6

DM93

Non-irradiated

98%

100%

25%

98%

90%

90%

100%

100%

UV

98%

100%

< 1%

5%

70%

85%

100%

100%

Gamma

100%

100%

30%

98%

80%

85%

100%

100%

UV + Gamma

98%

100%

< 1%

1%

75%

85%

100%

100%

  1. * Melanoma cells were non-irradiated, gamma-irradiated (200 Gy), UV-irradiated (5 min), or both gamma-irradiated (200 Gy) and UV-irradiated (5 min).
  2. †The percent of cells staining for gp100 expression, tyrosinase, MART-1/MelanA, and S100 expression was assessed by immunohistochemistry.