Figure 6
An in vivo study of TS siRNA in ACC3 tumor xenografts. 3 × 106 ACC3 cells were injected subcutaneously into the right flank of mice. When the tumors reached a volume of approximately 100 mm3, TS siRNA and negative control siRNA were injected to the tumors. TS and control siRNA were mixed with atelocollagen. Mice were sacrificed 2 weeks after siRNA injection. (C) After treatment with each siRNA, the xenografts were collected, fixed with 10% formalin, sectioned and stained for apoptotic cells by TUNEL method. The brown stain represented the DNA fragmentation of apoptotic cells and the blue stain showed the nuclei stain with hematoxylin (original magnification ×400). (D) The cumulative results showed the average number of apoptotic cells. Each group consisted of six mice. The values are represented as mean ± SD. *, P < 0.01, significantly different from control.