Figure 2

TS expression suppressed by TS-specific siRNA in ACC3 cells. After transfection for 48 h, TS expression was measured by conventional RT-PCR (A), real-time quantitative RT-PCR (B) and Western blot (C). Effect of TS suppression on cell survival in ACC3 cells (D). Cell survival conditions were evaluated by Cell Counting Kit-8 after transfection for 48 h. TS-suppressed cells were less proliferative than control cells (p < 0.01). The results are expressed as mean ± SD. *, P < 0.01, significantly different from control.