Gene expression variation between distinct areas of breast cancer measured from paraffin-embedded tissue cores

BMC Cancer

Table 2 Gene expression assays used for QRT-PCR

Symbol	Gene description	Accession No.	Category	Amplicon Size
GUSB	glucuronidase, beta	NM_000181.1	Control	81
RPLP0	ribosomal protein, large, P0	NM_053275.3 NM_001002.3	Control	105
UBB	ubiquitin B	NM_018955.2	Control	120
BCL2	B-cell CLL/lymphoma 2	NM_000633.2	ER	81
ESR1	estrogen receptor 1	NM_000125.2	ER	62
PGR	progesterone receptor	NM_000926.3	ER	118
SCUBE2	CEGP1, signal peptide, CUB domain, EGF-like 2	NM_020974.1	ER	64
ERBB2	v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)	NM_001005862.1 NM_004448.2	Her2	120
GRB7	growth factor receptor-bound protein 7	NM_005310.2	Her2	70
AURKA	STK15 aurora kinase A	NM_003600.2	Proliferation	85
BIRC5	baculoviral IAP repeat-containing 5 (survivin)	NM_001012271.1 NM_001168.2	Proliferation	93
CCNB1	cyclin B1	NM_031966.2	Proliferation	104
MKI67	antigen identified by monoclonal antibody Ki-67	NM_002417.3	Proliferation	131
MYBL2	v-myb myeloblastosis viral oncogene homolog (avian)-like 2	NM_002466.2	Proliferation	81

Shown are genes on the TLDA that were used for computing the PRO, ER and HER2 scores and the three control genes used for normalization [8].

ISSN: 1471-2407