Figure 3From: Cytoplasmic CXCR4 expression in breast cancer: induction by nitric oxide and correlation with lymph node metastasis and poor prognosis CXCL12 induced the chemoinvasion of breast cancer cells. Cells were seeded into the upper compartments of Matrigel Invasion Chambers as described in Materials and Methods. Uncoated or Matrigel-coated membranes separated the upper from the lower compartment containing the indicated concentrations of CXCL12. Cells were treated with DETA NONOate with or without L-NAME, and two days later, cells that had migrated to the bottom of the membrane were stained and counted. The percentage of invasive cells (% Invasion) was calculated as the number of cells penetrating the Matrigel-coated membranes divided by the number penetrating the uncoated membranes. Determinations were performed in triplicate and expressed as the mean of three experiments ± SD. * indicates significant difference (p < 0.05) from L-NAME-treated cells.Back to article page