Figure 6

Effect of calphostin C on MUC2 and NF-κB p65 expression. SEG-1 cells were treated for 18 hours with or without 100 μM DCA in the presence or absence of 5 nM calphostin C. (a) Total cellular and nuclear protein were subjected to Western blotting for MUC2, NF-κB p65, and β-actin. (b) SEG-1 cells were transfected with MUC2 promoter Luciferase construct or NF-κB Luciferase reporter plasmid, then treated for 18 hours with or without 100 μM DCA in the presence or absence of 5 nM calphostin C. Luciferase activity for MUC2 or NF-κB was measured and normalized to beta-galactosidase activity. (means ± SD of triplicate assays, * p < 0.05, for inhibition compared with assays without added inhibitor).