Figure 5

Effect of aspirin on MUC2 and Nuclear factor-κB (NF-κB) p65 expression. (a) SEG-1 cells were treated for 18 hours with or without 100 μM DCA in the presence or absence of 4 mmol/L aspirin. Total cellular and nuclear protein were subjected to Western blotting for MUC2, NF-κB p65, and β-actin. (b) SEG-1 cells were transfected with human MUC2 promoter luciferase construct or NF-κB luciferase reporter plasmid, then treated for 18 hours with or without 100 μM DCA in the presence or absence of 4 mmol/L aspirin. Luciferase activity for MUC2 or NF-κB was measured and normalized to beta-galactosidase activity. Values shown represent the means ± SD of triplicate experiments.