Figure 1

Effect of bile acids on MUC2 expression. (a) SEG-1 cells were treated for 18 hours with 50–300 μM DCA (left) or with 100 μM DCA, CDCA, or TCA (right). Total cellular protein was isolated and subjected to Western blotting for MUC2 and β-actin. (b) SEG-1 cells were transfected with MUC2 promoter luciferase construct, then treated for 18 hours with 50–300 μM DCA (left) or with 100 μM DCA, CDCA, or TCA (right). Luciferase activity for the MUC2 reporter was measured and normalized to beta-galactosidase activity. Values shown represent the means ± SD of triplicate experiments.