Identification of a highly recurrent chromosomal gain on 17q25.3 in prostate tumors by transcript co-regulation analysis. A) Ideogram of chromosome 17 detailing the genes located on 17q25.3 with a corregulated overexpression in prostate cancer, as determined by our microarray analysis (red: genes selected by FADA as overexpressed in tumor samples; black: genes not selected by FADA but present in the Human Genome Focus microarrays; grey: genes not present in Human Genome Focus microarrays). B) Heat map showing the relative expression levels of the genes on 17q25.3 shown in (A). The order of the genes is from centromeric (top) to telomeric (bottom). Fluorescent in situ hybridization of prostate tissue samples (C, D and E) hybridized with the centromeric CEP17 probe (green) and the 17q25.3-specific BAC clone RP11-165M24 (red). Selected representative regions of prostate carcinoma (C) and PIN (D) areas from the same sample, and a lymph node metastasis (E), illustrating copy number gains in 17q25.3. The figures shown are also representative of samples with a 17q25.3 gain.