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Table 3 Sequence of the mutation-specific TaqMan probes and PCR primers for the EGFR gene

From: Frequently increased epidermal growth factor receptor (EGFR) copy numbers and decreased BRCA1 mRNA expression in Japanese triple-negative breast cancers

Mutation No.

Probe name

Nucleotide

Amino Acid

Primer sequence (forward)

 

WT1

  

CCCAGAAGGTGAGAAAGTTAAAATTC

1

Del 1a

2235-2249del

E746-A750del

(same as above)

2

Del 1b

2236-2250del

 

(same as above)

3

Del 2

2254-2277del

S752-I759del

(same as above)

 

WT2

  

(same as above)

4

Del 3

2239-2247del, 2248G>C

L747-E749del, A750P

(same as above)

5

Del 4

2240-2257del

L747-S752del, P753S

(same as above)

6

Del 5

2238-2255del, 2237A>T

L747-A750del, E746V

(same as above)

7

Del 6

2240-2251del

L747-A750del, T747S

(same as above)

 

WT3

  

TGAGGATCTTGAAGGAAACTGAATTC

8

G719C

2155G>T

G719C

(same as above)

9

G719S

2155G>A

G719S

(same as above)

 

WT4

  

CCGCAGCATGTCAAGATCAC

10

L858R

2573T>G

L858R

(same as above)

 

WT5

  

(same as above)

11

L861Q

2582T>A

L861Q

(same as above)

 

WT6

  

CCCAGAAGGTGAGAAAGTTAAAATTC

12

L747Del

2239-2262del

L747-K754del

(same as above)

13

N756Ins

2265-2267InsCAA

N756Ins

(same as above)

 

WT7

  

GGAGGACCGTCGCTTGGT

14

L838P

2513T>C

L838P

(same as above)

TaqMan probe

Primer sequence (reverse)

   

VIC-ATTAAGAGAAGCAACATCT

CCCACACAGCAAAGCAGAAA

   

FAM-CGCTATCAAAACATCT

(same as above)

   

FAM-CTATCAAGACATCTCC

(same as above)

   

FAM-AGAAGCAACACTCGAT

(same as above)

   

VIC-CGAAAGCCAACAAG

(same as above)

   

FAM-CAAGGAACCAACATC

(same as above)

   

FAM-AAGGAATCGAAAGCC

(same as above)

   

FAM-CAAGGTTCCGAAAGC

(same as above)

   

FAM-TCAAGGAATCATCTCC

(same as above)

   

VIC-AAGTGCTGGGCTCC

TGCCAGGGACCTTACCTTATACA

   

FAM-AAAGTGCTGTGCTCC

(same as above)

   

FAM-AAAGTGCTGTGCTCC

(same as above)

   

VIC-TTGGGCTGGCCAAA

TCCTTCTGCATGGTATTCTTTCTCT

   

FAM-TTGGGCGGGCCAA

(same as above)

   

VIC-CCAAACTGCTGGGTG

(same as above)

   

FAM-CCAAACAGCTGGGTG

(same as above)

   

VIC-ATTAAGAGAAGCAACATCT

CCCACACAGCAAAGCAGAAA

   

FAM-CTATCAAGGAAGCCAACAA-MGB

(same as above)

   

FAM-CCAACAACAAGGAAAT-MGB

(same as above)

   

VIC-CGCGACCTGGCAG-MGB

CCCAAAATCTGTGATCTTGACATG

   

FAM-CGCGACCCGGCAG-MGB

(same as above)

   
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BMC Cancer

ISSN: 1471-2407

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