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Figure 2 | BMC Cancer

Figure 2

From: Glioma stem cells are more aggressive in recurrent tumors with malignant progression than in the primary tumor, and both can be maintained long-term in vitro

Figure 2

Expression of cell surface markers of tumor spheres before and after differentiation (×400). A-C, tumor spheres before differentiation. D-I, one week after FBS-induced differentiation. A, D, G, immunofluorescence staining for nestin (green). B, immunofluorescence staining for CD133 (red). E, immunofluorescence staining for GFAP (red). H, immunofluorescence staining for β-tubulin-III (red). C, F, I, confocol microscopy showed co-expression of nestin with CD133, GFAP and β-tubulin-III, respectively.

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