Figure 2
From: STAT3 can be activated through paracrine signaling in breast epithelial cells
Secretion of VEGF, IL-10, and IL-6 cytokines by various breast cell lines. A. VEGF secretion by breast cell lines after 24 hours. VEGF production does not correlate with p-STAT3 levels. B. IL-10 secretion by breast cell lines after 24 hours. IL-10 production does not correlate with p-STAT3 levels. C. IL-6 secretion by breast cell lines after 48 hours. p-STAT3-positive cell lines secrete high levels of IL-6 and cell lines that do not express constitutive p-STAT3 have low levels of IL-6 secretion. Cell lines that do not express constitutive p-STAT3: MDA-MB-453 and MCF-10A; p-STAT3-positive cells: MDA-MB-468, MDA-MB-231, and breast cancer associated fibroblasts (BCF).