Nestin expression in osteosarcomas and derivation of nestin/CD133 positive osteosarcoma cell lines

BMC Cancer

Table 2 Immunostaining of osteosarcoma-derived cell lines

Expression of nestin, CD133 and other proteins was examined on cell cultures of osteosarcoma-derived cell lines using indirect immunofluorescence. With the exception of the nestin-negative OSA-05 cell line, all cell lines showed even immunoreactivity for the detected antigens. Percentage of positive cells in cell populations (%: -, 0%; +/-, <2 %; +, 2–10 %; ++, 11–50 %; +++, 51–100 %) and intensity of immunostaining (IR: -, none; +, weak; ++, medium; +++, strong) were evaluated. Saos-2 cell line was originally used as a control osteosarcoma cell line for the CD133 staining.

ISSN: 1471-2407