Nestin expression in osteosarcomas and derivation of nestin/CD133 positive osteosarcoma cell lines

BMC Cancer

Table 1 Immunohistochemical analysis of nestin expression in osteosarcomas

Sample	Gender	Age	Tumor type		Nestin		Cell line
				% TC	IR TC	IR EC
1	F	8	C-OS-OB/DC	++	++	+
2	F	14	C-OS-OB	+	+/-	+/-
3	F	56	C-OS-OB	+	+	+/-
4	M	57	C-OS-OB	+	+/-	+/-
5	F	18	C-OS-OB	+	+/-	+/-
6	M	15	C-OS-OB	++	++	+
7	M	15	C-OS-OB	+	+	+/-
8	F	23	C-OS-CB/DC	+	+	+
9	M	21	C-OS-CB/DC	+	+/-	+/-
10	F	14	C-OS-OB	+/-	+/-	+/-
11	F	13	C-OS-OB	+	++	++	OSA-01
12	F	19	C-OS-OB	+	+	++
13	M	21	C-OS-OB	+++	+++	++
14	M	15	C-OS-OB	++	++	+
15	F	28	C-OS-OB	++	+	+/-
16	M	21	C-OS-OB	+++	+++	++	OSA-02
17	M	15	C-OS-OB	++	++	++	OSA-03
18	M	9	OS-TAE	+/-	+	+	OSA-05

Expression of nestin was examined on formalin-fixed, paraffin-embedded tissue specimens of osteosarcomas using immunohistochemistry. % TC, percentage of nestin positive tumor cells (+/-, <2 %; +, 2–10 %; ++, 11–50 %; +++, 51–100 %). IR TC, intensity of immunostaining (immunoreactivity) in tumor cells (+/-, very weak; +, weak; ++, medium; +++, strong). IR EC, intensity of immunostaining (immunoreactivity) in endothelial cells (+/-, very weak; +, weak; ++, medium; +++, strong). Tumor type: C-OS-OB, high grade conventional osteosarcoma, osteoblastic; OS-TAE, teleangiectatic osteosarcoma; C-OS-CB, high grade conventional osteosarcoma, chondroblastic; DC, decalcified.

ISSN: 1471-2407