|
First step(5'—3')
|
Second step(5'—3')
|
Product Size(BP)
|
---|
Exon18
|
F: ccagcactgacccaccac
|
F: ccagcactgacccaccac
|
228
|
|
R: ctcttgcccctcccatca
|
R: *agaactgccgaccacacc
| |
Exon19
|
F: cccacgctcttctcactcat
|
F: cccacgctcttctcactcat
|
183
|
|
R: agagaccagagcccagacct
|
R: *gggtccttcctgtcctccta
| |
Exon20
|
F: tgtggtctcccataccctct
|
F: *ctctcagcgtacccttgtcc
|
230
|
|
R: caaagagcccaggtgcatac
|
R: caaagagcccaggtgcatac
| |
Exon21
|
F: tacatgggtgcttcccattc
|
F: tacatgggtgcttcccattc
|
201
|
|
R:catgggctagacaccactcc
|
R: *gctccttggtccttcaccta
| |
Exon22
|
F: tagcccatgggagaactctg
|
F: *ctccccacaacacacagttg
|
186
|
|
R: agctctcatcctccctccag
|
R: agctctcatcctccctccag
| |
Exon23
|
F: actcctgaccctgtctctgc
|
F: actcctgaccctgtctctgc
|
220
|
|
R: ctttcatgccccttgtgg
|
R: *aggacctcccaccctcct
| |
Exon24
|
F: accagactggagggggagt
|
F: *agaggcagcaagcacacag
|
203
|
|
R: gagggtgctcttagccacag
|
R: gagggtgctcttagccacag
| |
- HER2-neu primers used for amplification (hemi-nested PCRs)
- * indicates that a GC clamp was added at the 5' end of the primer,
- GC clamp: cgcccgccgcgccccgcgcccggcccgccgcccccgcccg