Figure 5

Effect of Triphala is not cell specific. A) Effect of Triphala was evaluated on the survival of BxPC-3 (mutated p53) pancreatic cancer cell line, as described in Method section. B) Effect of ERK inhibitor on Triphala induced apoptosis. Cells were pretreated with 20 μM U0126, for 1 hour followed by exposure to 80 μg/ml Triphala for 4 h in the presence of inhibitor. C) Effect of Triphala on the survival of HPDE-6, normal human pancreatic ductal epithelial cells. D) Transcriptional activity of p53 in HPDE-6 cells treated with Triphala for 24 h as described in Method section. E) Effect of Triphala on the activation of ERK, p53, caspase-3 and PARP in HPDE-6 cells. The blots was stripped and reprobed with anti-actin antibody to ensure equal protein loading. These assays were performed 2–3 times independently and obtained similar results. Statistical significance was determined by one-way ANOVA followed by Bonferroni's post hoc analysis for comparisons.