Antiproliferative and apoptosis-inducing effects of Triphala in human pancreatic cancer cell line Capan-2. A) Cells were cultured in the presence of varying concentrations of Triphala (5–100 μg/ml) for 24 hours. Cell proliferation was measured by Sulphorhodamine B assay with eight replicates per Triphala concentration. Results of data are derived from three independent experiments and expressed as percent survival of Triphala treated cells compared to that of PBS treated control cells. B) Cells were treated with 20, 40 and 60 μg/ml Triphala for 24 hours. Cytoplasmic histone-associated DNA fragments were determined using cell death detection kit. The data represents mean ± SD of two independent experiments with 3 replicate in each experiment. C) Cells were treated with 20–60 μg/ml Triphala 24 hours. Subsequently cell lysates were prepared and 60 μg total protein was subjected to sodium dodecyl-polyacrylamide gel electrophresis followed by immunoblot analyses. The expression of caspase-9, caspase-3 and PARP were detected using appropriate antibodies. Blot was stripped and reprobed with anti-actin antibody to ensure equal protein loading. These experiments were performed 2–3 times independently, with similar results obtained in each experiment. *Statistically different compared with PBS-treated control (P < 0.05).