Table 1 Sequences of oligonucleotide primers used in real-time PCR and sequencing reactions
Primer pairs used in real-time PCR reactions | |
|---|---|
Gene | Primer sequence |
ACTB | Forward: 5'-TGCGTTACACCCTTTCTTGACA |
| Â | Reverse: 5'-GCAAGGGACTTCCTGTAACAATG |
GAPDH | Forward: 5'-GAAGGTGAAGGTCGGAGTC |
| Â | Reverse: 5'-GAAGATGGTGATGGGATTTC |
MT-ATP6 | Forward: 5'-TAGCCATACACAACACTAAAGGACGA |
| Â | Reverse: 5'-GGGCATTTTTAATCTTAGAGCGAAA |
MT-CO1 | Forward: 5'-GACGTAGACACACGAGCATATTTCA |
| Â | Reverse: 5'-AGGACATAGTGGAAGTGAGCTACAAC |
MT-CYB | Forward: 5'-ATCACTCGAGACGTAAATTATGGCT |
| Â | Reverse: 5'-TGAACTAGGTCTGTCCCAATGTATG |
MT-ND1 | Forward: 5'-CCACCTCTAGCCTAGCCGTTTA |
| Â | Reverse: 5'-GGGTCATGATGGCAGGAGTAAT |
MT-ND6 | Forward: 5'-CAAACAATGTTCAACCAGTAACCACTAC |
| Â | Reverse: 5'-ATATACTACAGCGATGGCTATTGAGGA |
MT-RNR1 | Forward: 5'-TAGAGGAGCCTGTTCTGTAATCGAT |
| Â | Reverse: 5'-CGACCCTTAAGTTTCATAAGGGCTA |
UBC | Forward: 5'-ATTTGGGTCGCGGTTCTTG |
| Â | Reverse: 5'-TGCCTTGACATTCTCGATGGT |
Primers used in sequencing reactions | |
Primer name | Primer sequence |
D1F A | Forward: 5'-AATGGGCCTGTCCTTGTAG |
D2F | Forward: 5'-CGACATCTGGTTCCTACTTC |
D3F | Forward: 5'-CGCTTCTGGCCACAGCAC |
L16112F | Forward: 5'-CACCATGAATATTGTACGGT |
D2R | Reverse: 5'-GGGTTTGGTTGGTTCGGG |
D3R A | Reverse: 5'-GGTGTGGCTAGGCTAAGC |
DLP4R | Reverse: 5'-GTGGAAAGTGGCTGTGCAG |
H16220R | Reverse: 5'-TTGATTGCTGTACTTGCTTGTAAG |
H16540R | Reverse: 5'-GTGGGCTATTTAGGCTTTATGACCCTG |