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Figure 4 | BMC Cancer

Figure 4

From: Characterization of cells recovered from the xenotransplanted NG97 human-derived glioma cell line subcultured in a long-term in vitro

Figure 4

GFAP and vimentin immunocytochemistry through cell subcultivation. Notice the negative modulatory expression pattern to GFAP (A – D) concomitant to the positive modulatory pattern to vimentin (E-H) in NG97(ht). The GFAP appears intensely marked in 100% of the 19th cell passage cytoplasm (A), being presented in approximately 85% of the 30th (B) and, finally, totally absent in 51st (C) and 80th (D) cell passages. Nonetheless, the vimentin were absent at 21st (E) cell passage, presented in weakly areas in the 40th (F) and in 54% of the 59th (G) cell passage and, finally, markedly positive at 83rd (H) cell passage (Original magnification: A – C and E – G = 200× and D and H = 1000×) (Positive and negative controls were assayed but were not displayed here).

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