Figure 3

Western Blot of NG97(ht) from different cell passages subcultivation to (A) GFAP and (B) vimentin, which contained approximately 5 μg and 30 μg of protein cytoplasm fraction, respectively. Lines 1, 2 and 3 show 23^rd, 33^rd e 95^th cell passages, respectively. (A) Note the GFAP basal linear conformational protein expression banded at approximated 50 kDa molecular weight in all passages. (B) Note the vimentin linear conformational basal expression banded at approximated 50 kDa molecular weight at earlier and later passages, though a 41,8 kDa molecular weight banded the 33^rd cell passage resulted from a partial vimentin degradation by the protein sonication extraction procedure.(Positive and negative controls were assayed but were omitted here).