Figure 2

Expression of VEGF, Rb, and p27 in cervical tissues. Panel A shows VEGF expression in the negative control cervical tissue that was evident mostly in the basal layer of the epithelium (100×). VEGF expression was seen in the small vessels as well as in the cytoplasm of the dysplastic epithelium cells, particularly in the upper and intermediate layers in CIN I (panel B) and in CIN II/III (panel C),(100×). Panel D shows a squamous cell carcinoma showing a diffuse cytoplasmic positivity for VEGF in the neoplastic cells (100×). In panel E the strong nuclear expression of Rb is evident in CIN I, localizing mainly to the basal layer of the epithelium (200×). Panel F shows p27 nuclear staining in the superficial layer of the epithelium, mainly in the koilocytes of a CIN I lesion (200×). Panel G shows HPV DNA detection by in situ hybridization in the CIN II/III HPV TMA case (400×). Panel H shows a low magnification view of several Tissue Micro-Array cervical samples used in the present study (20×).