Figure 4

Analysis of losses in 8p23.3. (a) Losses between 3.7 Mb and the telomere. Black bar, normal copy number for 8p; Grey, heterozygous loss; White with black outline, homozygous loss. Black arrows, position of primer pairs that amplify a product from MIA PaCa-2 genomic DNA i.e. lie outside the homozygous deletion; Grey arrows, position of primer pairs that do not amplify a product from MIA PaCa-2 genomic DNA. (b) Sequencing trace showing the point mutation in exon 19 of ARHGEF10. The normal sequence has a cytosine at the position indicated by the arrow. (c) Conservation of ARHGEF10. Yellow, the residue that is converted from a histidine to tyrosine by the point mutation in DU4475; Grey, conserved residues.