Identification of biomarkers in ductal carcinoma in situ of the breast with microinvasion

BMC Cancer

Table 2 Details of immunohistochemistry and the antibodies

Antibody	Source	Clone	Dilution	Pretreatment	Citrate buffer	Incubation	Detection system
ER	DAKO	1D5	1/50	4 × 15 min MW	pH6	1 h RT	ABC
PgR	DAKO	636	1/800	4 × 15 min MW	pH6	1 h RT	ABC
HER2	DAKO		1/300	4 × 15 min MW	pH6	1 h RT	ABC
Ki-67	DAKO	MIB-1	1/50	3 × 10 min MW	pH9	1 h RT	ABC
Bcl-2	DAKO	124	1/50	3 × 10 min MW	pH9	ON 4°C	En-Vision
Bax	DAKO		1/50	3 × 10 min MW	pH6	ON 4°C	En-Vision
Survivin	SantaC	D-8	1/50	3 × 10 min MW	pH9	1 h RT	ABC
p53	DAKO	DO-7	1/50	3 × 10 min MW	pH9	1 h RT	ABC
p21	DAKO	SX118	1/25	3 × 10 min MW	pH9	1 h RT	ABC
cyclinD1	NovoC	P2D11F11	1/50	3 × 10 min MW	pH9	1 h RT	ABC
Rb	NovoC	13A10	1/100	3 × 10 min MW	pH6	1 h RT	ABC

ER = estrogen receptor; PgR = progesterone receptor; MW = microwave; RT = room temperature; ON = overnight; Santa C = Santa Cruz; NovoC = NovoCastra.

ISSN: 1471-2407