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Table 2 Details of immunohistochemistry and the antibodies

From: Identification of biomarkers in ductal carcinoma in situ of the breast with microinvasion

Antibody Source Clone Dilution Pretreatment Citrate buffer Incubation Detection system
ER DAKO 1D5 1/50 4 × 15 min MW pH6 1 h RT ABC
PgR DAKO 636 1/800 4 × 15 min MW pH6 1 h RT ABC
HER2 DAKO   1/300 4 × 15 min MW pH6 1 h RT ABC
Ki-67 DAKO MIB-1 1/50 3 × 10 min MW pH9 1 h RT ABC
Bcl-2 DAKO 124 1/50 3 × 10 min MW pH9 ON 4°C En-Vision
Bax DAKO   1/50 3 × 10 min MW pH6 ON 4°C En-Vision
Survivin SantaC D-8 1/50 3 × 10 min MW pH9 1 h RT ABC
p53 DAKO DO-7 1/50 3 × 10 min MW pH9 1 h RT ABC
p21 DAKO SX118 1/25 3 × 10 min MW pH9 1 h RT ABC
cyclinD1 NovoC P2D11F11 1/50 3 × 10 min MW pH9 1 h RT ABC
Rb NovoC 13A10 1/100 3 × 10 min MW pH6 1 h RT ABC
  1. ER = estrogen receptor; PgR = progesterone receptor; MW = microwave; RT = room temperature; ON = overnight; Santa C = Santa Cruz; NovoC = NovoCastra.