Table 1 Primer sequences and optimized annealing temperatures for each pair of primers
Primers | Sequence | CpG sites | Location | Length (bp) | Annealing temp. (°C) |
|---|---|---|---|---|---|
P1 | 5' TATGTAGGATCGAAGTTTTC 3' | 2 | -405 ~ -64 | 341 | 60–62 |
P2 | 5' TAAAAACGCCTCCGCCG 3' | 3 | Â | Â | Â |
P3 | 5' GGGTATAGTGCGGGAGC 3' | 2 | -235 ~ +22 | 257 | 65–67 |
P4 | 5' CAACCGCACGAAAAACGC 3' | 3 | Â | Â | Â |
P5 | 5' GAGGCGTTAGGCGGTTC 3' | 3 | -180 ~ -26 | 155 | 64.5 |
P6 | 5' AACTAACGACCGACGAAC 3' | 3 | Â | Â | Â |
U1 | 5' GAGGTGTTAGGTGGTTTGTT 3' | 3 | -180 ~ +71 | 252 | 62 |
U2 | 5' CCCACCAAAATCACAACAA 3' | 3 | Â | Â | Â |