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Figure 2 | BMC Cancer

Figure 2

From: TOR complex 2 is needed for cell cycle progression and anchorage-independent growth of MCF7 and PC3 tumor cells

Figure 2

Inhibition of TORC2 reduces proliferation of MCF7 and PC3 cells. (A) The amount of programmed cell death (PCD) was analyzed by calculating the percentage of cells displaying nuclear condensation 4 days after siRNA treatments. In the table the percentage of condensed nuclei and the number of cells analyzed is displayed. Note that MCF7 cells lack functional caspase-3, which makes them resistant and affects their mode of cell death [20]. When treated with the commonly used cell death inducer, staurosporin, MCF7 cells undergo nuclear condensation without the nuclear fragmentation typical of normal apoptotic cells (upper panel). (B) MCF7 and PC3 cells were plated at low density and transfected with two independent rictor siRNAs or with a control siRNA. Cells were allowed to proliferate for two days until they reached near confluency, they were trypsinized, diluted and replated at low density and retransfected with the siRNAs. Cells were allowed to proliferate another four days until the control cells were nearly confluent. Cells were trypsinized, resuspended and counted with a counting chamber. All samples were done in three replicates. * Students t-test p < 0.01.

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