Msi1 downstream effects. The RNA levels of a subset of Notch, Hedgehog and Wnt pathway components were quantified by quantitative RT-PCR in control and knockdown Daoy cells. Gene expression changes are represented (mean ± se) using semi-logarithmic scale. The plot in the upper part (a) shows the fold change in mRNA levels for the Msi1 knockdown normalized to the housekeeping gene 15S. The table (b) summarizes the ratio (in percentage) of mRNA levels detected in Msi1 knockdown vs control. * represents p < 0.05 and ** p < 0.01.