Msi1 expression in Daoy neurospheres. The levels of MSI1 RNA levels were analyzed in Daoy cells grown as monolayer (nonNSC) and as neurosphere (NSC) cultures using quantitative RT-PCR. GAPDH and 15S mRNA levels were used as endogenous control for normalization. On average, a 2-fold increase in MSI1 was detected in the neurosphere cultures.* indicate p < 0.01.