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Table 1 Main characteristics of the antibodies used in immunohistochemistry.

From: Markers of subtypes in inflammatory breast cancer studied by immunohistochemistry: Prominent expression of P-cadherin

Protein (Clone) Antibody Origin Clone Pre-treatment Dilution Location of staining Normal
Caveolin 1 mmb Santa Cruz N20 Micro waves (10 min), tps citrate pH = 6 1/1000 cytoplasm m.e.c+
Caveolin 2 mmb Transduction Laboratories 65 Target retrival solution (98°C, 40 min) 1/50 cytoplasm m.e.c+
Cytokeratin 5/6 mmb Dako D5/16 B4 Target retrival solution (98°C, 40 min) 1/10 cytoplasm m.e.c +
Cytokeratin 14 mmb Newcastle UK LL002 Target retrival solution (98°C, 40 min) 1/300 Cell membrane m.e.c+
EGFR mmb Zymed 3IG7 Target retrival solution (98°C, 40 min) 1/10 membrane m.e.c +
ERBB2 mmb Dako Herceptest Ltd AO485 Target retrival solution (98°C, 40 min) 1/500 membrane -
Estrogen receptor (ER) mmb Novocastra laboratories Ltd 6F11.2 Target retrival solution (98°C, 40 min) 1/60 nucleus m.e.c- and luminal+
P53 mmb Dako DO-1 Target retrival solution (98°C, 40 min) 1/4 nucleus -
P-cadherin mmb Transduction laboratories 56 Target retrival solution (98°C, 40 min) 1/75 Cell membrane m.e.c +
Progesterone receptor (PR) mmb Dako PFR 636 Target retrival solution (98°C, 40 min) 1/80 Nucleus m.e.c- and luminal+
Ki67 mmb Dako KI-67 Target retrival solution (98°C, 40 min) 1/100 Nuclear -
MUC1 mmb Transgen H23 none 1/1000 Apical/Cytoplamic +
  1. mmb: mouse monoclonal antibody
  2. m.e.c: myoepithelials cells