Table 1 Main characteristics of the antibodies used in immunohistochemistry.
Protein (Clone) | Antibody | Origin | Clone | Pre-treatment | Dilution | Location of staining | Normal |
|---|---|---|---|---|---|---|---|
Caveolin 1 | mmb | Santa Cruz | N20 | Micro waves (10 min), tps citrate pH = 6 | 1/1000 | cytoplasm | m.e.c+ |
Caveolin 2 | mmb | Transduction Laboratories | 65 | Target retrival solution (98°C, 40 min) | 1/50 | cytoplasm | m.e.c+ |
Cytokeratin 5/6 | mmb | Dako | D5/16 B4 | Target retrival solution (98°C, 40 min) | 1/10 | cytoplasm | m.e.c + |
Cytokeratin 14 | mmb | Newcastle UK | LL002 | Target retrival solution (98°C, 40 min) | 1/300 | Cell membrane | m.e.c+ |
EGFR | mmb | Zymed | 3IG7 | Target retrival solution (98°C, 40 min) | 1/10 | membrane | m.e.c + |
ERBB2 | mmb | Dako Herceptest Ltd | AO485 | Target retrival solution (98°C, 40 min) | 1/500 | membrane | - |
Estrogen receptor (ER) | mmb | Novocastra laboratories Ltd | 6F11.2 | Target retrival solution (98°C, 40 min) | 1/60 | nucleus | m.e.c- and luminal+ |
P53 | mmb | Dako | DO-1 | Target retrival solution (98°C, 40 min) | 1/4 | nucleus | - |
P-cadherin | mmb | Transduction laboratories | 56 | Target retrival solution (98°C, 40 min) | 1/75 | Cell membrane | m.e.c + |
Progesterone receptor (PR) | mmb | Dako | PFR 636 | Target retrival solution (98°C, 40 min) | 1/80 | Nucleus | m.e.c- and luminal+ |
Ki67 | mmb | Dako | KI-67 | Target retrival solution (98°C, 40 min) | 1/100 | Nuclear | - |
MUC1 | mmb | Transgen | H23 | none | 1/1000 | Apical/Cytoplamic | + |