A) Western blot analysis showing down-regulation of FABP7 in WM35 and WM239 cells after transfection with FABP7 siRNA, with α-tubulin as loading control. B-C) The effect of FABP7 down-regulation on proliferation in WM35 (B)and WM239 (C)cells measured by [3H]Thymidine incorporation 72 hrs post transfection. Down-regulation of FABP7 protein using siRNA led to reduction of DNA synthesis, suggesting reduced proliferation in both cell lines.D) Matrigel invasion assay. Inhibition of invasion ability of WM35 and WM239 cells following FABP7 down-regulation with siRNA. The average was calculated from three independent experiments and presented with standard deviation.