Figure 2From: CXCR4 expression in papillary thyroid carcinoma: induction by nitric oxide and correlation with lymph node metastasis CXCL12 induced the chemoinvasion of papillary thyroid cancer cells. Cells were seeded into the upper compartments of Matrigel Invasion Chambers as described in Materials and Methods. Uncoated or Matrigel-coated membranes separated the upper compartments from lower compartments containing the indicated concentrations of CXCL12. Cells were treated with DETA NONOate with or without L-NAME. Two days later, cells that had migrated to the bottom of the membrane were stained and counted. The percentage of invasive cells (% Invasion) was calculated as the number of cells penetrating the Matrigel-coated membranes divided by the number penetrating the uncoated membranes. Determinations were performed in triplicate and expressed as mean of three experiments ± SE. * indicates significant difference (p < 0.05) from L-NAME-treated cells.Back to article page