Table 2 SSCP conformation and haplotypic frequencies, clinical parameters and laboratory data of the NOS3 gene in peripheral blood of patients with prostate cancer and benign prostatic hyperplasia.
From: The -786T>C promoter polymorphism of the NOS3gene is associated with prostate cancer progression
PCa (N = 83) | BPH (N = 94) | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
SSCP Conformation | Genotypes(a) | N (%) | * Age | * PSA (ng/ml) | * TNM score | * Gleason score | % positive PCA3 | *ŧ [NOS3] | % (N) | * Age | * PSA (ng/ml) | % positive PCA3 | *ŧ [NOS3] |
TN | c. [-786T>C] + [=] c. [-690C>T] + [=] c. [-649G>A] + [=] | 1 (1.2) | 77 | 101.0 | -- | 9 | 0 (0 out of 1) | -- | 0 | -- | -- | -- | -- |
CC | c. [-786T>C] + [-786T>C] c. [-714G>T] + [-714G>T] c. [-690C>T] + [-690C>T] | 9 (10.8) | 67.5 (7.3) | 41.4 (78.8) | 1 | 8 | 42.9 (3 out of 7) | -- | 8 (8.5) | 66.8 (7.8) | 15.3 (11.7) | 12.5 (1 out of 8) | 0.5 (0.2) |
TT | No mutations | 30 (36.2) | 68.5 (8.4) | 24.0 (28.4) | 2 | 7 | 28.0 (7 out of 25) | 1.1 (1.8) | 29 (30.9) | 66.7 (9.2) | 9.8 (10.3) | 18.5 (5 out of 27) | 0.5 (0.3) |
TC | c. [-786T>C] + [=] c. [-714G>T] + [=] c. [-690C>T] + [=] | 32 (38.6) | 69.7 (7.8) | 17.3 (18.7) | 1 | 6 | 35.7 (10 out of 28) | 1.1 (1.5) | 35 (37.2) | 68.9 (8.4) | 11.9 (12.3) | 15.4 (4 out of 26) | 0.6 (0.4) |
TA | c. [-786T>C] + [=] c. [-764A>G] + [=] c. [-690C>T] + [=] | 4 (4.8) | 64.5 (10.0) | 11.6 (7.2) | 2 | 6 | 66.7 (2 out of 3) | 4.7 (4.3) | 13 (13.8) | 67.2 (10.1) | 9.5 (7.3) | 20.0 (2 out of 10) | 0.4 (0.3) |
CA | c. [-764A>G] + [=] c. [-714G>T] + [=] c. [-786T>C] + [-786T>C] c. [-690C>T] + [-690C>T] | 5 (6.0) | 63.8 (7.4) | 9.4 (3.4) | 1 | 6 | 40.0 (2 out of 5) | 6.0 (0.3) | 9 (9.6) | 66.7 (8.6) | 11.9 (7.9) | 42.9 (3 out of 7) | 0,6 |
AA | c. [-786T>C] + [-786T>C] c. [-764A>G] + [-764A>G] c. [-690C>T] + [-690C>T] | 2 (2.4) | 70.5 (7.8) | 12.1 (12.1) | -- | 7 | 50.0 (1 out of 2) | -- | 0 | -- | -- | -- | -- |
Total | 83 | 43.1 (25 out of 71) | 94 | 19.0 (15 out of 78) | |||||||||
p (1) | p = 0.0082 | p = 0.0011 | |||||||||||
p (2) | p = 0.10 | ||||||||||||
Haplotypes | % | * Age | * PSA (ng/ml) | * TNM score | * Gleason score | % positive PCA3 | *ŧ [NOS3] | % | * Age | * PSA (ng/ml) | % positive PCA3 | *ŧ [NOS3] | |
N | c. [-786T>C; -690A>G; -649G>A] | 1.0 | 77 | 101.0 | -- | 9 | 0.0 | -- | 0 | -- | -- | 0 | |
T | No mutations | 58.0 | 69.9 | 38.5 | 1 | 6 | 56.0 | 1.1 | 56.0 | 67.6 | 10.4 | 53.2 | 0.4 |
C | c. [-786T>C; -714G>T; -690C>T] | 33.0 | 66.7 | 22.7 | 1 | 7 | 32.0 | 1.1 | 32.0 | 67.5 | 11.0 | 30.1 | 0.3 |
A | c. [-786T>C; -764A>G; -690C>T] | 8.0 | 66.3 | 11.0 | 2 | 6 | 12.0 | 2.6 | 12.0 | 66.9 | 10.7 | 16.7 | 0.2 |
Total | 100.0 | 100.0 (25) | 100.0 | 100.0 (15) | |||||||||