Figure 3

Nested RT-PCR for the PCA3 and B2M gene expression analysis. A) Lanes 1 to 4: amplifications for the PCA3 gene, where sample pairs 1 and 2, 3 and 4, correspond to two RNA extraction replicates for each patient. The detection of the expected 277-bp PCA3 fragment evidences the presence of circulating tumor cells. Lane M: 100-bp size marker. B) Lanes 1 to 4: amplifications for the B2M gene (534-bp). Semi-quantitative RT-PCR for the NOS3 and B2M gene expression analysis. The 322-bp and 534-bp fragments correspond to the NOS3 and B2M genes, respectively. Lane M: 100-bp size marker. Lanes 1 to 10 represent individual patients, where (C) represent BPH patients' profile, and (D) the PCa patients' profile.