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Table 2 Oligonucleotides used to create point mutations in the hMYH-type1-Flag/pcDNA

From: A haplotype variation affecting the mitochondrial transportation of hMYH protein could be a risk factor for colorectal cancer in Chinese

Mutation

Oligos for fragments "a" and "b" in the 1st. PCR (5'→3') *

p.Pro18Leu- p.Gly25Asp

c.53C>T-c.74G>A

Fa:GGCGTGGATAGCGGTTTGA

Ra:CACTTCCCACGGCTGCTCGTaGCTTCCTCATGA

Fb: CGAGCAGCCGTGGGAAGTGaTCACAGGAAGCA

Rb: TGAAATTCCTCCTGCGTCAGC

p.Pro18Leu

c.53C>T

Fa:GGCGTGGATAGCGGTTTGA

Ra:CACTTCCCACGGCTGCTCGTaGCTTCCTCATGA Fb:TCATGAGGAAGCtACGAGCAGCCGTGGGAAGTG

Rb: TGAAATTCCTCCTGCGTCAGC

p.Gly25Asp

c.74G>A

Fa:GGCGTGGATAGCGGTTTGA

Ra: TGCTTCCTGTGAtCACTTCCCACGGCTGCTCG

Fb: CGAGCAGCCGTGGGAAGTGaTCACAGGAAGCA

Rb: TGAAATTCCTCCTGCGTCAGC

  1. *Three bolded letters in the sequence represent the code of a mutated amino acid.
  2. The lowercase letters are the substituted nucleotide residues.
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BMC Cancer

ISSN: 1471-2407

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