Figure 2

Differences in structure and subcellular localization between the wild- and variant-type hMYH proteins. (A) Schematic diagrams showed the structures of the wild-type hMYH type 1 protein (upper) and the hMYH protein with haplotype variation predicted a missense protein of p.Pro18Leu – p.Gly25Asp (lower). Dots represented the polymorphic amino acids, which was mapped to the N-terminus of the MTS domain. (B) The distinct subcellular localization of the wild- and variant-type of hMYH proteins. The wild-type (a) and variant-types (b, c, d) hMYH with the FLAG epitope tag at the C-terminus were expressed in cos-7 cells and stained with anti-FLAG M2 as the first antibody and FITC-conjugated anti-mouse IgG as the second antibody. The immunofluorescence microscopic image of FITC (green)-stained cells showed the localization of exogenous hMYH protein. The mitochondrias were counterstained with CMXros (red). The merged FITC and PI stained images showed overlapping yellow signals in the mitochondrias. Only the hMYH protein with haplotype variation (b) presented dual localization in nuclei and mitochondrias.