Figure 1

Identification of haplotype variation c.53C>T-c.74G>A in hMYH gene. (A) DHPLC chromatogram of exon 2 of hMYH gene showed the wild type and the mutant pattern. The arrow was pointed to the aberrant peak in patients with variant. (B) Partial sequence of exon 2 of hMYH gene (forward sequencing). Direct sequencing depicted 2 nucleotide substitutions (c.53C > T and c.74G > A). (C) Sequencing of cloned PCR product revealed the 2 nucleotide substitutions C > T and G > A on the same allele. Arrows showed the mutant sites.