Figure 8
From: Restoration of tumor suppressor miR-34 inhibits human p53-mutant gastric cancer tumorspheres
Restoration of miR-34 in Kato III cells delays cell growth. Kato III cells were infected with feline immunodeficiency virus (FIV) lentiviral system expressing miR-34a (miR-34a-MIF) or vector control (MIF), and stable cells were obtained by antibiotic selection (Zeocin 50 μg/mL) and validated for miR-34a expression. The stable cells were plated in a 24-well plate with equal cell density. Cells in triplicate were collected by trypsinization and viable cells were counted by Trypan Blue exclusion at 24-h intervals over 4 days, using a Coulter cell counter (Beckman). ***P < 0.001 versus MIF vector control, two-way ANOVA, n = 3.