Figure 6

Restoration of miR-34 in Kato III cells resulted in G1 block and caspase-3 activation. A. Cell cycle analysis of Kato III cells after miR-34 restoration. Cell cycle analysis was performed 24 hours after transfected with miR-34 mimics or negative control mimic (NC mimic). Cells were stained with propidium iodide after ethanol fixation and analyzed by flow cytometry. **P < 0.01, ***P < 0.001, versus that of NC mimic, one-way ANOVA, n = 2. B. Caspase-3 activation in Kato III cells after miR-34 restoration. 24 hours after transfection, cells were lysed for measurement with the Caspase-3 activation assay kit (BioVision). Relative increase of fluorescence signal was calculated by dividing the normalized signal in each treated sample with that of NC mimic as 100. **P < 0.01 versus that NC mimic, two-tailed t-test, n = 3.