Table 2 Paired primers used to amplify the specific sequence of ATBF1-A, including the partial mutation loci previously reported in prostate cancer
From: Loss of heterozygosity at the ATBF1-A locus located in the 16q22 minimal region in breast cancer
|  |  |  |  | Primer sequence (5'→3') |  | |
|---|---|---|---|---|---|---|
Exon | Mutation no. | Nucleotide* | Amino acid | Forward | Reverse | Product size (bp) |
9 | 1 | (5556–5559) del | Frameshift | AGCAACCGGTCAGCCAGAAC | GGTGGCATCCCTACACTCTCAG | 336 |
|  | 2 | 5602C→T | No change |  |  |  |
|  | 3 | (5891–5896) del |  | CTCCAAGCTCTAACTTACTAAGCCAA | AGTTGCAGCAGGGTCTCAGTT | 349 |
| Â | 4 | 5897insCAA | Gln1741ins | Â | Â | Â |
| Â | 5 | 6350delAG | Frameshift | AGCCAGCACCCCGAAAAGA | CTTGCCGCAGGAGTCACAC | 360 |
| Â | 6 | 6447delG | Frameshift | Â | Â | Â |
|  | 7 | 7154C→T | R2160W | CACCGGCCCAGCCATCAGT | AACCCGGACTTGTCTGCCATCT | 322 |
|  | 8 | 8364A→G | Gln2564Arg | TCAACTCCTCAACAGCTCGCAA | GCTTGCACTGGCCTTTTCCTC | 333 |
|  | 9 | 8919C→T | A2749V | CGCAGGCCCACAGGAGA | CAAAGTCTTCAATCCCTTCCACC | 321 |
|  | 10 | 9380A→G | S2903G | AACTCTTCTAAGCCCTTCCTCCAT | CACTGTAGTCCACTGTACCTTCATTG | 356 |
|  | 11 | 9434G→A | A2921T | CCGAGCTTTTATAGCAAGGAATATG | ACTTGGACTTCTTTTCTTTTGCCC | 322 |
10 | 12 | (10814–10834)del | 7 amino acid loss | TCCTTACTTTGTACCAGGCTTTTCTC | TCTTCTGGTTTGGGGGATTCTTTG | 345 |
|  | 13 | (10814–10837)del | 8 amino acid loss |  |  |  |
|  | 14 | (10826–10846)del | 7 amino acid loss |  |  |  |