Figure 2

2a. RNA response to molecular inhibitors. CD44 total and variant RNA in triplicate TaqMan RT-PCR experiments. In subconfluent G_sα-QL prostate cancer cells, significant decreases of about 50% or more were observed after protein kinase A (PKA) inhibitor H-89, or inhibitors of downstream signaling pathways MEK or p38 kinase, but not JNK inhibitor. Error bars are standard deviation. *p = 0.01; **p = 0.001; ^†p = 0.0002; ***p = 0.0001 with respect to mock treated controls. 2b. Western blot analysis. Protein kinase A inhibitor exerts a dose-dependent decrease on CD44s and CD44v compared with EtOH control in G_sα-QL cells.